ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescent quantum yield, and high photo-stability. Key antibody specifications include clonality, reactivity, host, and conjugation. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3 and is characterized by a high photostability. Commonly Used Fluorochromes and Their Properties The results from our flow cytometry, immunocytochemistry, and immunohistochemistry experiments demonstrate that protein-conjugated, long-wavelength Alexa Fluor dyes have advantages compared to the Cy dyes and other long-wavelength dyes in typical fluorescence-based cell labeling applications. Images were taken as two or four channel images at a . Here we show that the calcium channel antagonist nimodipine significantly attenuated clinical disease and central nervous system degeneration and also fostered remyelination in a mouse model of MS. More Options for Multicolor Flow Cytometry General Information 300 350 400 450 500 550 600 650 700 750 800 850 100 80 60 40 20 0 More choice BD continues to provide more choices for multicolor flow cytometry applications by expanding our portfolio and color options. - amine
S. Yeou, N. Lee, Contribution of a DNA Nick to DNA Bendability Depending on the Bending Force, Bulletin of the Korean Chemical Society 42, 1151 (2021). Additional file 4: Figure S4 Internalization of FITC-Insulin and BAC-Ins-SA-atto 550. BD FACS Aria II Fluorochromes PMT A PMT B Hoechst (red for SP) (675/50 BP) BUV 395 (379/28 BP) Hoechst (blue for SP) (450/50 BP) Hoechst for viability (450/50 BP) Looks like you're visiting us from {{countryName}}. PDF Introduction to flow cytometry - Abcam The program is subject to the conditions of use of the manufacturer. R. Ankri, A. Basu, A. Ulku, C. Bruschini, E. Charbon, S. Weiss, X. Michalet, Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium, ACS Photonics 7, 68 (2019). Kaminski et al., Light-inducible molecular beacons for spatiotemporally highly defined activation, Chem. S. Zirkin, S. Fishman et al., Lighting Up Individual DNA Damage Sites by In Vitro Repair Synthesis, J.