5.0 g/mL Methylene Blue Working Solution. Watch Video 1 on how to perform a serial dilution using "pour plates"and the associated calculations: Watch video 2 on how to perform a serial dilution and make spread plates. In the first example above, we wanted to dilute a sample with an incredibly high bacterial concentration to an easily countable number of bacterial cells, so diluted 1 ml of the original sample or previous dilution in 9 ml of fresh diluent. Measure _______ g of solid sodium acetate in a weigh boat on an electronic balance. During that time, each individual viable bacterial cell multiplies to form a readily visible colony. All rights reserved. Therefore, there were 241 X 102 CFU/mL in the original sample or expressed 2.41 x 104. Moreover, it will allow you to get more accurate and precise results, as the pre-wetting steps, pipetting speeds, mixing cycles and mixing volumes will always be consistent. Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes in the laboratory. This is nice and well understood. Explain how that gas is dissolved in water. Label the bottle with the solution with the following information: The name of the solution (include concentrations), Write a fraction for the concentration \[5\:\%\: ( \frac{w}{v} )\: =\: \dfrac{5\: g\: sucrose}{100\: mL\: solution} \nonumber\], Set up a proportion \[\dfrac{5\: g\: sucrose}{100\: mL\: solution} \:=\: \dfrac{?\: g\: sucrose}{500\: mL\: solution} \nonumber\], Solve for g sucrose \[\dfrac{5\: g\: sucrose}{100\: mL\: solution} \: \times \: 500 \: mL \: solution \: = \: 25 \: g \: sucrose \nonumber\]. Give an example of two liquids demonstrating the law of multiple proportions. If you have a 1:3 dilution, i.e., a 1:3 dilution ratio, this means that you add 1 unit volume of solute (e.g., concentrate) to 3 unit volumes of the solvent (e.g., water), which will give a total of 4 units of volume. Serial Dilution: Importance and Aplication - HubPages However, because the mixture in the first tube is not perfectly uniform, it could be that you only transfer 298 bacterial cells. What is the OH- of this sample? Tea or coffee are two common examples of serial dilution that occur in everyday life. The dilution ratio is the ratio of the solute (the substance to be diluted) to the solvent (e.g., water).The diluted liquid needs to be thoroughly mixed to achieve true dilution. Due to the period decrease in concentration, this method is very useful when performing many types of experiments, from chemistry to biology to medicine.You can plot the information they provide onto a graph to find the gradient and intercepts (or calculate them with our slope intercept form calculator), so that information about .