We postulate that IHF mediates the formation of a higher order structure between the divergent promoter regions in a manner analogous to the nucleosome-like structure generated for lambda-Escherichia coli DNA recombination and that this higher order structure modulates transcription. PleA is also required for the assembly of substructures of the flagellar basal body hook complex that are located in or traverse the peptidoglycan layer. This finite window of opportunity is imposed by coordinating spatially constrained proteolysis of CtrA, an inhibitor of DNA replication initiation, with forward progression of the cell cycle. Like Dam in the enterobacteria, CcrM plays a regulatory role in Caulobacter crescentus and Rhizobium meliloti. A series of simple, in situ immunoassays have been developed which can be used in screening for translation products of genes cloned in vitro recombination experiments with either phage or plasmid vectors. Under conditions which inhibited DNA synthesis but permitted phospholipid synthesis, i.e., growth of a temperature-sensitive DNA elongation mutant at the restrictive temperature or treatment with hydroxy-urea, stalk elongation occurred normally. We also seek to systematically explore the role of AKAP79/150 in orchestrating transcriptional and regulatory control of M/KCNQ channels in sympathetic and sensory neurons. WEISSBORN, A., Steinman, H. M., Shapiro, L. SYNTHESIS OF SPECIFIC MEMBRANE-PROTEINS IS A FUNCTION OF DNA-REPLICATION AND PHOSPHOLIPID-SYNTHESIS IN CAULOBACTER-CRESCENTUS. Since the phage is extremely salt-sensitive, a purification procedure was devised which avoided contact with solutions of high ionic strength. The SMC foci appear randomly distributed in the cell. x@caltech.edu, x=ltorress, Alumni Analysis of the cloned and sequenced dnaK gene has shown that the deduced amino acid sequence could encode a protein of 67.6 kilodaltons that is 68% identical to the DnaK protein of Escherichia coli and 49% identical to the Drosophila and human hsp70 protein family. Surprisingly, in this one-micron bacterial cell, the dynamic spatial disposition of regulatory proteins, structural proteins and specific regions of the chromosome are important components of both cell-cycle progression and the generation of daughter cells with different cell fates. SMC complexes and histone-like proteins continuously remodel the nucleoid to reconcile chromatin compaction with DNA replication and gene regulation. Using a cosmid library we isolated a clone that complemented SC1130. Many predivisional cells have bright polar SMC foci, which are lost upon cell division. Following cell division, only the chromosome in the progeny stalked cell is able to initiate DNA replication, while the chromosome in the progeny swarmer cell does not replicate until later in the cell cycle. In ultrasound gradients, GVs and cells expressing them get pushed more strongly and in the opposite direction from other biological materials. The occurrence of the recognition sequence for an essential DNA methylating enzyme that is required for cell cycle regulation is severely limited and shows a bias to intergenic regions. We found that the extent to which MreB localization is perturbed is linearly correlated with the development of pointed cell poles and variable cell widths. Moreover moving the native ctrA gene to a position near the chromosomal terminus, which delays the conversion of the ctrA promoter from the fully to the hemimethylated state until late in the cell cycle, inhibited ctrA P1 transcription, and altered the time of accumulation of the CtrA protein and the size distribution of swarmer cells. View details for Web of Science ID A1997WQ86300029, View details for PubMedCentralID PMC178970, View details for Web of Science ID A1997WM41300002. 1986 Fudan University B., McAdams, H. H., Shapiro, L., Collier, J.